CAMAG AMD 2 System Automated Multiple Development

The separation of complex samples is a challenging task for every chromatographic system, particularly when the sample components span a wide polarity range. The AMD procedure offers an excellent solution as it allows stepwise gradient elution over increasing separation distances. As a result acids, bases, neutral, hydrophilic, and lipophilic substances can be separated in a single AMD run. This makes AMD suitable for a variety of applications. The technique is frequently used in lipid analysis and in routine analysis of drinking water. Pigment formulations with a complex composition, resins as well as additives of mineral oil products are other typical applications of AMD analysis.

The principle of the CAMAG AMD 2 procedure:
1) The HPTLC plate is developed repeatedly in the same direction;
2) Each successive run extends over a longer solvent migration distance than the one before;
3) Between runs, the solvent is completely removed from the developing chamber and the layer is dried under vacuum;
4) Each successive run uses a solvent of lower elution strength than that of the one used before. In this way, a stepwise elution gradient is formed;
5) The combination of focusing effect and gradient elution results in extremely narrow bands. Their typical peak width is about 1 mm. This means that, with the available separation distance of 80 mm, up to 40 components can be completely resolved, i.e. with base line separation.

Key Features:
• Multiple development
• Stepwise gradient elution over increasing separation distances
• Enhanced separation capacity
• HPTLC glass plates (20x10 cm), only
• Software-controlled with visionCATS

You can purchase additional accessories to CAMAG AMD 2:
022.8880 Oil-sealed rotary vane vacuum pump RV3 (Edwards)
028.0000 CAMAG HPTLC Software visionCATS Basic Version