Fluorescence Spectroscopy

Fluorescence spectroscopy is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light.

A unique feature of fluorescence, distinguishing it from other spectroscopic techniques, is its inherently multidimensional character. Fluorescent properties of every compound are characterized by two basic types of spectra: excitation and emission, while not all of the absorbing molecules are fluorescent. These features contribute to higher selectivity of fluorescence as opposed to absorption spectra. Fluorescence spectroscopy offers a very sensitive window into the structural and dynamic characteristics of macromolecules.

Fluorescence spectroscopy is used in biochemical, medical, and chemical research fields for analyzing organic compounds.